Plasmid_Backbone

Part:BBa_K1641999:Design

Designed by: Chunyang Ni   Group: iGEM15_SYSU_CHINA   (2015-09-11)


pAUR135 shuttle vector RFC10 optimized


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 5858
    Illegal suffix found at 5880
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5858
    Illegal NheI site found at 2517
    Illegal NheI site found at 2796
    Illegal SpeI site found at 5881
    Illegal PstI site found at 5895
    Illegal NotI site found at 5864
    Illegal NotI site found at 5888
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5858
    Illegal BamHI site found at 2975
    Illegal BamHI site found at 5844
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 5858
    Illegal suffix found at 5881
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 5858
    Plasmid lacks a suffix.
    Illegal XbaI site found at 5873
    Illegal SpeI site found at 5881
    Illegal PstI site found at 5895
    Illegal AgeI site found at 4808
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1197
    Illegal BsaI.rc site found at 2857
    Illegal BsaI.rc site found at 5411
    Illegal SapI site found at 4246
    Illegal SapI.rc site found at 2278


Design Notes

mutation and MCS modification


Source

DNA synthesis

References